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SRX5546575: GSM3680530: CC12h: sampling C control 12h; Carica papaya; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 15.5M spots, 3.1G bases, 1,005.9Mb downloads

Submitted by: NCBI (GEO)
Study: Transcriptome profile of papaya ripening
PRJNA528193 • SRP188930 • All experiments • All runs
show Abstracthide Abstract
We report the application of NGS-derived transcriptome profile to elucidate a temporal line of both control and ethylene-induced ripening process of papaya fruit focusing on ripening-induced transcripts that act directly on plant cell wall disassembling Overall design: Fruits were collected at 3 different times of the year (biological triplicate) and treated with 100-ppm ethylene for 12 hours and then let to ripe for 12 hours more. In this way, it was created five groups of analyses: control 0hrs, 12hs and 24hs and ethylene-treated fruits 12hs and 24hs.
Sample: CC12h: sampling C control 12h
SAMN11175661 • SRS4512193 • All experiments • All runs
Organism: Carica papaya
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: The total RNA of the fruits pulp was isolated using Concert ™ Plant RNA Reagent (Invitrogen®) and purified by treatment with the DNA-free ™ kit (Invitrogen®), following the protocol described by the manufacturer. Libraries were prepared using the Illumina TruSeq Stranded mRNA LT Sample Prep Protocol
Experiment attributes:
GEO Accession: GSM3680530
Links:
Runs: 1 run, 15.5M spots, 3.1G bases, 1,005.9Mb
Run# of Spots# of BasesSizePublished
SRR875586115,466,4783.1G1,005.9Mb2021-09-07

ID:
7477375

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